PCR and Genetic Engineering

From UBC Wiki

DNA Replication

Explain the logic of the process of DNA replication including how the cell solves the problems of: separating the DNA strands, replicating both strands simultaneously, providing primers for laggings strand DNA synthesis, replicating the ends of the DNA, explain what an Okazaki fragment is and its role in replication

PCR and Genetic Engineering

Describe how restriction enzymes can cut DNA at specific sites

Describe how restriction enzymes and plasmids can be used to clone DNA sequences and make many copies of them in bacteria

Describe the relationship between the length of a DNA double helix and its temperature stability and relate this to the specificity of binding of an oligonucleotide (a primer) to a DNA molecule

Describe the unusual properties of how DNA polymerases begin synthesis of DNA

Describe the process of polymerase chain reaction amplification of DNA including the role of each step

Be able to locate where primers should be placed to amplify a given region of DNA

Accurately predict the products of an amplification reaction given locations of primers on a DNA