DNA Replication
Explain the logic of the process of DNA replication including how the cell solves the problems of: separating the DNA strands, replicating both strands simultaneously, providing primers for laggings strand DNA synthesis, replicating the ends of the DNA, explain what an Okazaki fragment is and its role in replication
PCR and Genetic Engineering
Describe how restriction enzymes can cut DNA at specific sites
Describe how restriction enzymes and plasmids can be used to clone DNA sequences and make many copies of them in bacteria
Describe the relationship between the length of a DNA double helix and its temperature stability and relate this to the specificity of binding of an oligonucleotide (a primer) to a DNA molecule
Describe the unusual properties of how DNA polymerases begin synthesis of DNA
Describe the process of polymerase chain reaction amplification of DNA including the role of each step
Be able to locate where primers should be placed to amplify a given region of DNA
Accurately predict the products of an amplification reaction given locations of primers on a DNA
