Prokaryotic gene regulation-lac operon
Explain the logic of the function and regulation of the lac operon relative to the availability of lactose
Draw a correct representation of the lac operon that includes the lacZYA genes, the promoter and operator regions, the lacI gene with its promoter
Draw a correct representation of which proteins are bound to the control regions of the lac operon based on the presence and absence of glucose and lactose
Predict the effects of mutations in the DNA sequences of the lac operon and the genes and promoters for LacI
Explain the mechanisms of repression of lac operon
Describe what is meant by basal level transcription and how the cell regulates this transcription
Use the lac operon example to explain other examples of regulation
Explain the logic of the function and regulation of the mal operon relative to the availability of maltose
Maltose Operon
Draw a correct representation of the mal operon that includes the genes, the promoter and operator regions, and the malT gene with its promoter
Draw a correct representation of MalT bound to the control regions of the mal operon based on the presence and absence of maltose
Predict the effects of mutations in the malT gene on the amount of Mal enzymes produced
Use the mal operon example to explain other examples of transcription regulation
Contrast the mechanisms of positive and negative regulation of operons
Eukaryotic gene expression
Describe how the combination of the GAL4 regulatory protein and promoter proximal elements in yeast coordinate expression of galactose catabolism genes
Be able to apply the concepts of positive and negative regulation to regulation of galactose catabolism genes
Compare and contrast regulation of the Lac and Mal operons in bacteria to the regulation of galactose catabolism genes in yeast
