Documentation:Standard Operating Procedures for Water Bath

1.  Please obtain proper training from the FNH teaching lab technician Imelda Cheung (Imelda.Cheung@ubc.ca) prior to using the water bath.

2.  Check to make sure there is sufficient CLEAN water in the water bath. If the water looks dirty or cloudy, drain the water and re-fill with ddwater.

Only ddwater is used in water bath!

3.  Turn on the water bath and set the desired temperature digitally or by adjusting the dial. Do not EXCEED the temperature limits of the water bath or it will be damaged.

Sheldon Model 1212: 20 – 80 ^oC (above 60 ^oC require lid on)

Fisher Isotemp 120: 20 – 100 ^oC (above 60 ^oC require lid on)

Boekel Grant PB-2800: 5 – 99 ^oC (above 60 ^oC require lid on)

Fisher Water Bath: 20 – 80 ^oC (above 60 ^oC require lid on)

If unsure, ASK FOR HELP!

4.  Once set, the water bath cycles on and off to ensure constancy of the temperature.

It is strongly recommended that you place a thermometer in a flask in the bath to obtain real-time temperature of the bath.

5.  When the temperature stabilized and the heat light goes off, the water bath is ready to use. You may now place your samples in the water bath.

If using temperature above 50 ^oC, be sure to use tongs or oven mitts to retrieve samples to avoid scalding.

6.  When experiment is complete, remove all your samples from the bath, and turn off the water bath.

If you spill any sample in the bath, wait till the bath cools to below 40 ^oC, then discard all the water to the nearest sink and notify the FNH teaching lab technician for sanitizing. Failure to do so will result in

forbidden usage of the teaching lab facility.