Documentation:Standard Operating Procedures for Accumet AB15 pH Meter

Accumet AB15 pH meter

1. Please obtain proper training from the FNH teaching lab technician Imelda Cheung (Imelda.Cheung@ubc.ca) prior to using the pH meter.

   • A video https://www.youtube.com/watch?v=lL1cYcwfJFo is also provided for review purpose and can be viewed as needed.

2. Turn on pH meter by pressing “Stdby”.
3. Press “Setup” to access the clear pH standardization menu.
4. Press “Setup” again to display clear buffer icon.
5. Press “enter” to clear all existing buffers.
6. Press “Setup” to access pH Buffer Group 2, 4, 7, 10, 12.Press “enter” to accept the group.
7. Remove pH probe carefully from storage bottle and turn the probe to “ON” position.
8. Rinse with ddH₂O and gently dry with kimwipes.
9. Immerse the probe in pH 4 buffer and press “std”.
10. When value stabilized, press “std” to recognize the buffer.
11. Repeat steps 8 – 10 for pH 7 and 10 buffers.
12. The percent slope displayed at the end of calibration should be within the range of 90 – 102%.

    Please note that when you are in standardization mode, the LCD will display ‘standardize’. When standardization is complete, the LCD will display ‘read’.

13. The electrode is now ready for use to read samples.
14. When pH measurement is complete, rinse the probe thoroughly with ddH₂O, dry with kimwipes, then place the probe carefully back into the storage bottle filled with pH 4 buffer.

    If you note that the buffer level in the storage bottle does not soak the pH probe, please add pH 4 buffer to the bottle. pH 4 buffer can be found under shelf “P” in MCML 230G.

15. Turn the probe to “OFF” position.

    It is important to close the probe or else electrolyte will evaporate and the probe may be damaged.

16. Turn off pH meter by pressing “Stdby”.

To reserve a time to utilize the pH meters, please visit https://www.qreserve.com/r/resource/QR00182946