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Summary
| Description | English: 1. Centrifuge the CSF for 10-15 minutes at 1000 x g and collect the supernatant. The sediment should be used for Gram stain and primary culture.
2. Heat the CSF supernatant to be used for the test at 100°C for 3 minutes. 3. Shake the latex reagents gently until homogenous. 4. Place one drop of each latex reagent on a disposable card provided in the kit or a ringed glass slide. 5. Add 30-50 μl of the supernatant of the CSF to each latex reagent. 6. Rotate by hand for 2-10 minutes. If available, mechanical rotation at 100 rpm is recommended. • Avoid cross-contamination when mixing and dispensing reagents. 7. Examine the agglutination reactions under a bright light without magnification. (Centre of Disease Control and Prevention, 2015) |
| Date | 23 February 2015 () |
| File source | http://webpath.med.yale.edu/microbio/microbe/microbe05.htm |
| Author | Edward C. Klatt MD |
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| current | 05:01, 24 February 2015 | | 600 × 391 (10 KB) | WaldenCheung (talk | contribs) | User created page with UploadWizard |
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