Gene regulation in prokaryote and eukaryote

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Prokaryotic gene regulation-lac operon

Explain the logic of the function and regulation of the lac operon relative to the availability of lactose

Draw a correct representation of the lac operon that includes the lacZYA genes, the promoter and operator regions, the lacI gene with its promoter

Draw a correct representation of which proteins are bound to the control regions of the lac operon based on the presence and absence of glucose and lactose

Predict the effects of mutations in the DNA sequences of the lac operon and the genes and promoters for LacI

Explain the mechanisms of repression of lac operon

Describe what is meant by basal level transcription and how the cell regulates this transcription

Use the lac operon example to explain other examples of regulation

Explain the logic of the function and regulation of the mal operon relative to the availability of maltose

Maltose Operon

Draw a correct representation of the mal operon that includes the genes, the promoter and operator regions, and the malT gene with its promoter

Draw a correct representation of MalT bound to the control regions of the mal operon based on the presence and absence of maltose

Predict the effects of mutations in the malT gene on the amount of Mal enzymes produced

Use the mal operon example to explain other examples of transcription regulation

Contrast the mechanisms of positive and negative regulation of operons

Eukaryotic gene expression

Describe how the combination of the GAL4 regulatory protein and promoter proximal elements in yeast coordinate expression of galactose catabolism genes

Be able to apply the concepts of positive and negative regulation to regulation of galactose catabolism genes

Compare and contrast regulation of the Lac and Mal operons in bacteria to the regulation of galactose catabolism genes in yeast