Taman Nasional Karimunjawa secara geografis terletak pada koordinat 5°40’39”- 5°55’00” LS dan 110°05’ 57”-110°31’ 15” BT. Dalam Surat Keputusan Menteri Kehutanan dan Perkebunan No. 78/Kpts-II/1999 tanggal 22 Februari 1999 dinyatakan bahwa kawasan Cagar Alam Karimunjawa dan sekitarnya yang terletak di Kabupaten Dati II Jepara Propinsi Dati I Jawa Tengah ditetapkan menjadi Taman Nasional dengan nama TAMAN NASIONAL KARIMUNJAWA dengan luasan kawasan adalah 111.625 hektar
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