The System Water

The S11 spotter requires two kinds of water. A system water, that fills system tubings and is used to flush the nozzle, and a wash water that is used to fill the wash station, and washes the exterior of the nozzle. The system water is fed to the system through a media bottle in front of the pump station. The wash water is fed to system through a carboy that sits on the ground, underneath the bench. A second carboy located next to the wash water carboy is used to collect the waste solution.

There are different requirements for each these water sources:

System Water

• The system water needs to be free from particles, to avoid clogging the nozzle and the tubes, and as sterile as practically possible, to avoid biofilm growth in the system tubings.
• It is crucial to use freshly filtered water everyday, and to discard the remainder of the water at the end of the day. The remainder of the system water can be discarded into the wash water carboy for sustainability.
• The followings, are suitable waters for the system water:
  • DI water obtained from DI water container in bioroom, AND filtered using the Millipore Simplicity water purification system.
  • DI water obtained from DI water container in bioroom, AND filtered using 0.22 um Millipore Striltop vacuum bottle top filters (Fisher Scientific, Cat # SCGPT05RE)
  • UNOPENED Sigma sterile-filtered BioReagent cell culture-grade water (Sigma-Aldrich Cat # W3500-24X500ML)

Wash Water

• The followings, are suitable waters for the system water:
  • DI water obtained from DI water container in bioroom.
  • Unused System Water at the end of the day.