Nuclei and chromatin

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Biological membranes
Unit 3 BIOL 200
Welcome to the tutoring wiki for BIOL 200. Here is where you'll find learning objectives and summary for Unit 3 of BIOL 200
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Interphase Nucleus

Describe the structure and organization of the components of the interphase nucleus, and recognize these structures in TEM micrographs.

Explain how proteins are transported into and out of the nucleus, including the roles of the nuclear localization/export signal, the importin/exportin receptors of the NLS, and the nuclear pore complex.

Interpret experiments that show how nuclear localization signals may be necessary and sufficient for nuclear import.

Nucleic Acids Review

Recognize the structures of nucleotides, purines, pyrimidines, ribose, deoxyribose.

Distinguish the 3' and 5' ends of a DNA molecule.

Explain the role of hydrogen bonds in generating secondary structure of RNA.

Chromatin and Chromosomes

Analytically describe chromatin structure. Explain how proteins and DNA interact to form chromosomes, starting with the 2nm naked DNA molecule.

Discuss primary, secondary, tertiary, quaternary levels of protein structure in the context of chromatin structure.

Explain the structure of nucleosomes and the forces stabilizing these structures.

Explain further condensation of the chromatin structure, including the 11 nm fibre, higher order structures, chromosomal loops, euchromatin and heterochromatin.

Diagram the major parts of chromosomes including the centromere and telomere.


Explain and interpret experiments providing quantitative information about the spacing of nucleosomes and the amount of DNA associated with these structures.